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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and therefore might lead to tumorigenesis. This protein was found to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a significant role in selling cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are explained. Two added splice variants were being reported but in-depth nucleotide sequence details is not really nevertheless available.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized with the antibody.
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This guide outlines even further feasible optimization of cellular and immunoassay parameters to ensure the very best outcomes are...
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This guide outlines even further achievable optimization of cellular and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide no more bind into the epitope within the goal protein. By comparing the staining with the blocked antibody vs . the antibody by itself, one can see which staining is certain.
Goal Information and facts Cyclin E belongs on the extremely conserved cyclin spouse and children, whose users are characterized by a remarkable periodicity in protein abundance throughout the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins show unique expression and degradation patterns which lead on the temporal coordination of every mitotic occasion. Cyclin E forms a fancy with and functions for a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates on the G1-S section boundary and is also degraded as cells progress through S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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